1. Tank System:

Tank material: S31603 or S30408 stainless steel; Tank design pressure: 0.3MPa; Jacket design pressure: 0.35MPa;

Tank structure: Equipped with sampling and discharging valves for fermentation tanks, and standard temperature, pH, and DO sensor ports;

Can lid structure: 1 standard foam electrode socket; 1 sight glass opening; 3 universal feeding interfaces; 1 inoculation port; 1 vent port;

Surface treatment: Stainless steel inner and outer mirror polishing, polishing precision Ra0.4;

2. Sterilization method: In-situ steam sterilization;

3. Stirring system: Three-layer, six-blade standard impeller for fermentation; control range: 50~300rpm, control accuracy: ±1%;

4. Temperature control: Cooling water temperature +5~65℃; Accuracy: ±0.1℃;

5. pH control: Mettler pH electrode and shielded wires from Switzerland: range: 2~12 pH, control accuracy: ±0.02 pH;

6. Dissolved oxygen control: Mettler DO electrode and shielded wires (Swiss): Range: 5~100%, control accuracy: ±2%;

7. Foam control: Automatic foam detection and defoamer addition: sensitivity 100~100000;

8. Feeding control: Automatic feeding via peristaltic pump; range: 0~5L/min, control accuracy: ±2%;

9. Air flow control: Manual adjustment of the rotor flow meter; range: 0~5000L/min;

Overview

A bio-fermentation tank is a device for mechanically stirring and fermenting materials. This device adopts an internal circulation method and uses a stirring paddle to disperse and break up bubbles. It has a high oxygen dissolution rate and good mixing effect. The tank body is made of SUS304 stainless steel and is equipped with an automatic spray cleaning head to ensure that the production process complies with pharmaceutical GMP standards.

Configuration

Material inlet/outlet, coolant inlet/outlet, liquid level display interface, temperature sensor interface, pH meter interface, pressure sensor interface, air respirator interface, CIP interface, defoaming electrode interface, flow meter interface, instrument control system.

structure

The main components of a bio-fermentation tank include the tank body, agitator, baffles, air distributor, cooling pipes (or jacket), defoamer, manhole, and integrated sight glass.

This series of fermenters consists of a seed fermenter, mounted on a platform. The two tanks can be used separately, and fermentation process parameters can be controlled independently. It is suitable for microbial fermentation, scale-up, and pilot-scale experiments, as well as for optimizing fermentation process parameters and testing production processes and strains. It is an ideal piece of equipment for microbiology laboratories and secondary pilot-scale fermentations in research institutes and enterprises.

Complete set of engineering equipment for bio-fermentation cell culture

(1) Stirring method: Standard mechanical stirring system

(2) Features:

Nominal volume: 100L~150000L

Height-to-diameter ratio: H:D = 2.0~3.0

Loading coefficient: 0.7

Sterilization method: In-situ sterilization system configuration: Generally, a three-stage fermentation process is adopted, with a seed tank to fermenter ratio of 1:10.

System parameter control: A three-level fermentation control system is provided according to user needs, including seed tank, fermentation tank, control system, air filtration system, valve platform, utilities, etc.

Process flow diagram

Main technical parameters